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Updated: Sep 26, 2026
Real-Time Polymerase Chain Reaction-Based Detection and Quantification of Hepatitis B Virus DNA
Published on: December 15, 2023
[Detection of IgM-antibody to hepatitis G virus and its clinical significance]
Objective:
To provide a rapid and early serodiagnostic technique for the patients with hepatitis G virus(HGV).
Methods:
A capture ELISA was developed to detect anti-HGV IgM in sera using synthetic peptides of HGV NS3 NS5 gene regions conjugated with horseradish peroxidase.
Results:
This assay was not disturbed by competition of specific IgG or interfered by RF and did not cross-react with HAV, HBV, HCV, HEV, CMV and EBV. Among 46 sera of non-A-E hepatitis, 14 were positive for anti-HGVIgM, the positive rate was 30.43%, 6 was positive for HGV RNA the positive rate was 42.86%. 12 paired sera from acute stage of HGV patients were all positive by capture-ELISA.
Conclusion:
This method is sensitive, specific, rapid and stable for detecting anti-HGV IgM, and is useful in the early diagnosis of HGV infection.
