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Updated: Sep 25, 2026
Homogeneous Time-resolved Förster Resonance Energy Transfer-based Assay for Detection of Insulin Secretion
Published on: May 10, 2018
On-line SPE-RP-LC for the determination of insulin derivatives in biological matrices
N F C Visser1, H Lingeman, H Irth
1Department of Analytical Chemistry and Applied Spectroscopy, Division of Chemistry, Faculty of Sciences, Vrije Universiteit Amsterdam, de Boelelaan 1083, 1081 HV Amsterdam, The Netherlands. lingeman@chem.vu.nl
Abstract:
An automated and on-line solid-phase extraction (SPE)-liquid chromatography (LC) procedure is described for the determination of insulin in biological matrices. The total procedure consists of two SPEs in series, followed by RP-LC separation. During the first SPE a strong anion-exchange (SAX) cartridge (ISOLUTE, 40-90 microm, 10 x 4 mm i.d.) is used, followed by a RP-cartridge (Luna C(8), 4 x 2.0 mm i.d.). The second SPE cartridge contains the same material as the LC column and is used to transfer the sample from the SAX cartridge to the LC column. The developed system can detect 100 nmol/l insulin in aqueous samples and 200 nmol/l insulin in spiked plasma samples using UV. When electrospray ionization (ESI)-mass spectrometry (MS), was coupled with the developed system, the LODs were lowered by a factor two to 50 and 100 nmol/l for aqueous and spiked plasma samples, respectively.
