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Assembly and Purification of Prototype Foamy Virus Intasomes

Assembly and Purification of Prototype Foamy Virus Intasomes

Published on: March 19, 2018

Polydnavirus integration in lepidopteran host cells in vitro.

D E Gundersen-Rindal¹, D E Lynn

¹US Department of Agriculture, Agricultural Research Service, Insect Biocontrol Laboratory, Beltsville, MD 20705, USA. gundersd@ba.ars.usda.gov

Journal of Insect Physiology

|May 29, 2003

Summary

Polydnavirus (PDV) DNA integrates into gypsy moth cell genomes, evidenced by junction clones. This viral DNA integration in vitro suggests a host-derived mechanism, not viral proteins.

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Area of Science:

Virology
Genetics
Insect molecular biology

Background:

Polydnavirus (PDV) DNA exhibits long-term persistence in infected lepidopteran cell cultures.
This persistence suggests potential permanent integration into the host cell genome.

Purpose of the Study:

To provide supportive evidence for the in vitro integration of polydnavirus DNA into lepidopteran cell genomes.
To identify precise integration sites and characterize the mechanism of polydnavirus integration.

Main Methods:

Preparation of cloned libraries from Lymantria dispar cell lines infected with Glyptapanteles indiensis PDV (GiPDV).
Isolation of junction clones containing both insect chromosomal and viral sequences.
Sequence comparison of linear and circular forms of GiPDV genome segment F to identify integration junctions.

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Main Results:

Identified junction clones with both insect and GiPDV sequences in L. dispar cell lines.
Determined identical virus sequence junctions with a CATG palindromic repeat, despite variable host chromosomal sequences.
Observed a novel L. dispar retrotransposon near the integration site, suggesting a host-derived integration mechanism.

Conclusions:

Polydnavirus DNA integrates into the L. dispar cell genome in vitro.
Integration appears to be mediated by a host-derived mechanism, potentially involving retrotransposon activity.
Further research is needed to determine if this integration occurs in vivo within the lepidopteran host.