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Integrated capillary fluorescence DNA biosensor.

Marc A Breimer1, Yevgeny Gelfand, Omowunmi A Sadik

  • 1Department of Chemistry, State University of New York at Binghamton, PO Box 6016, Binghamton, NY 13902-6016, USA.

Biosensors & Bioelectronics
|June 6, 2003
PubMed
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This study describes a new method for attaching double-stranded DNA (dsDNA) to glass capillaries, creating a biosensor. This biosensor effectively detects fluorescent analytes and metal ions like nickel and lead.

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Materials Science

Background:

  • Developing sensitive and specific biosensors is crucial for detecting various analytes.
  • Immobilization of biomolecules onto solid supports is a key step in biosensor development.
  • Existing methods for DNA immobilization can be complex and may require hybridization.

Purpose of the Study:

  • To develop a novel method for covalent attachment of double-stranded DNA (dsDNA) onto glass capillaries.
  • To create a biosensor substrate for detecting fluorescent organic analytes and metal ions.
  • To characterize the immobilization efficiency and binding capacity of the dsDNA-modified surface.

Main Methods:

  • Covalent immobilization of dsDNA molecules directly onto the inner surface of glass capillaries.

Related Experiment Videos

  • Characterization of surface density and specific binding capacity of immobilized dsDNA.
  • Fabrication of a biosensor utilizing a capillary-geometry flow cell.
  • Testing the biosensor's response to fluorescent dyes and metal ions (Ni2+, Pb2+).

    • Main Results:

    • Achieved a high surface density of dsDNA (2.5 x 10^13 molecules/cm^2) with a specific binding capacity of 62.5%.
    • Demonstrated successful retention of fluorescent dyes on the dsDNA-modified surface.
    • Observed a reproducible decrease in fluorescence intensity upon exposure to nickel and lead ions, indicating metal binding.

    Conclusions:

    • Covalent attachment of dsDNA within glass capillaries offers an efficient immobilization strategy without hybridization.
    • The developed dsDNA-modified capillary serves as a robust platform for a novel biosensor.
    • The biosensor shows promise for detecting fluorescent analytes and metal ions with high specificity and reproducibility.