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Free Radicals in Chemical Biology: from Chemical Behavior to Biomarker Development
Published on: April 15, 2013
Development of chemo-enzymatic process and manufacture of deoxynucleosides
H Komatsu1, H Awano, H Ishibashi
1Chemical Synthesis Laboratory, Mitsui Chemicals Inc., 1144 Togo, Mobarashi, Chiba 297-0017, Japan.
Abstract:
Manufacturing method for 2'-deoxynucleosides (dNus) has been developed using novel chemo-enzymatic process. The method consists of the chemical synthesis of 2'-deoxyribose 1-phosphate (dRP) and the enzymatic conversions of dRP into 2'-deoxyadenosine (dA), 2'-deoxyguanosine (dG) and 2'-deoxycytidine (dC). Large-scale preparation is successfully done, which demonstrates an applicability of this strategy to practical use. As thymidine is already under commercial-scale production, all four dNus are now ready for scale-up.
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