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Reverse transcriptional mutagenesis induced by ribonucleoside triphosphate analogue, PTP and its availability for
K Moriyama1, T Okada, D Loakes
1Gene Research Center, Okayama University, 1-1-1 Tsushima-naka, Okayama 700-8530, Japan.
Nucleic Acids Research. Supplement (2001)
|July 3, 2003
Abstract:
The bicyclic pyrimidine analogue, 3,4-dihydro-6H,8H-pyrimido[4,5-c][1,2]oxazin-7-one (P) can base pair with both A and G. The riboside 5'-triphsophate of P (PTP) efficiently induces mutation during in vitro transcription and reverse transcription cycles using a phage promoter. In the present study, we have constructed an in vitro transcription system promoted by the human immunodeficiency virus type 1 (HIV-1) 5'-long terminal repeat (LTR) using HeLa nuclear extract supplemented with HIV-1 Tat protein. Using this system, the effects of mutagenic ribonucleotide analogues were studied.