Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hydrogenases in sulfate-reducing bacteria function as chromium reductase.

B Chardin1, M-T Giudici-Orticoni, G De Luca

  • 1Unité de Bioénergétique et Ingénierie des Protéines, UPR 9036, CNRS-IBSM, 31 chemin Joseph Aiguier, 13402 Marseille cedex 20, France.

Applied Microbiology and Biotechnology
|July 16, 2003
PubMed
Summary

Sulfate-reducing bacteria (SRB) enzymes, particularly [Fe] hydrogenase, effectively reduce toxic chromate VI for environmental remediation. This study highlights hydrogenases as key players in chromate reduction, offering promising bioremediation strategies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Distal arthrogryposis with impaired proprioception and touch: description of 9 additional cases harbouring novel PIEZO2 variants and literature review.

European journal of paediatric neurology : EJPN : official journal of the European Paediatric Neurology Society·2026
Same author

A scaffold for quinone channeling between membrane and soluble bacterial oxidoreductases.

Nature structural & molecular biology·2025
Same author

Comparative effectiveness and cost-effectiveness of natalizumab and fingolimod in rapidly evolving severe relapsing-remitting multiple sclerosis in the United Kingdom.

Journal of medical economics·2023
Same author

Drug development and novel therapeutics to ensure a personalized approach in the treatment of systemic sclerosis.

Expert review of clinical immunology·2023
Same author

PD-L1 expression, BRAF and TERT mutation in a cohort of aggressive thyroid cancers: case series from a single-centre experience.

Journal of endocrinological investigation·2023
Same author

Corrigendum to "Impact of hemoglobin levels at admission on outcomes among elderly patients with acute coronary syndrome treated with low-dose Prasugrel or clopidogrel: A sub-study of the ELDERLY ACS 2 trial" [Int J Cardiol. 2022 Dec 15;369:5-11].

International journal of cardiology·2023

Area of Science:

  • Environmental Microbiology
  • Bioremediation
  • Biocatalysis

Background:

  • Sulfate-reducing bacteria (SRB) possess mechanisms for chromate VI reduction, crucial for environmental decontamination.
  • Chromate VI reduction can occur chemically via H(2)S or enzymatically through polyheme cytochromes c(3).

Purpose of the Study:

  • To investigate the role of various hydrogenases from SRB in chromate VI reduction.
  • To identify specific enzymes and metal centers involved in the detoxification of chromate VI.

Main Methods:

  • Isolation and characterization of hydrogenases ([Fe], [NiFe], [NiFeSe]) from Desulfovibrio and Desulfomicrobium species.
  • Enzyme activity assays, including kinetic studies (K(m)) and electron paramagnetic resonance (EPR) spectroscopy.
  • Analysis of mutant strains lacking specific hydrogenases to confirm in vivo activity.

Related Experiment Videos

Main Results:

  • Multiple hydrogenases, including [Fe], [NiFe], and [NiFeSe] types, exhibit chromate VI reductase activity.
  • [Fe] hydrogenase from Desulfovibrio vulgaris strain Hildenborough showed the highest reduction rate.
  • Cr(VI) is reduced to Cr(III), with enzyme inhibition observed at high chromate concentrations. Low redox [Fe-S] clusters are implicated in the reduction mechanism.

Conclusions:

  • Hydrogenases are significant contributors to chromate VI reduction by SRB, complementing other known mechanisms.
  • The [Fe] hydrogenase plays a critical role in vivo for chromate VI detoxification.
  • Understanding these enzymatic pathways provides a basis for developing bioremediation technologies for chromate-contaminated sites.