Related Experiment Video
Updated: Sep 23, 2026

Single-Molecule Analysis of Sf9 Purified Superprocessive Kinesin-3 Family Motors
Published on: July 27, 2022
Redistribution of motor proteins by Cytochalasin J treatment
Rollin W Robinson1, Judith A Snyder
1Department of Biological Sciences, University of Denver, 2190 E. Iliff Avenue, 80208, Denver, CO, USA.
Abstract:
In this study we extend our analysis of the effect of Cytochalasin J (CJ) on mitotic and interphase cells by the use of immunocytochemical techniques to localize antigens to anti-beta-tubulin, anti-dynein heavy chain (HC), anti-dynein intermediate chain (IC), and anti-kinesin antibodies following CJ treatment. Anti-dynein IC and HC staining of CJ treated cells showed a significant reduction in anti-dynein staining in the nuclear region of interphase cells. Monolayer cultures of PtK(1)cells treated with 10 microg/ml CJ for 10 min showed a significant reduction in pixel luminosity of fluorescence staining using anti-dynein IC and HC antibodies (P<0.05). Cytochalasin J treatment reorganized anti-dynein staining from a cytoplasmic punctate staining with greatest intensity in the perinuclear region, to a more uniform staining throughout the cytoplasm.
Related Concept Videos
Microtubule Associated Motor Proteins
The Movement of Organelles and Vesicles
Studying the Cytoskeleton
Destabilization of Microtubules
Anaphase A and B
Plus-end depolymerization releases tubulin heterodimers from the terminal region of the microtubule. As tubulin subunits are lost, the Ndc80 complexes detach...
Cell Motility through Blebbing
Blebbing Through the Matrix
In multicellular...

