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Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
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Oligonucleotides with strongly fluorescent groups pi-conjugated to a nucleobase: syntheses, melting temperatures, and

Guan-Sheng Jiao1, Kevin Burgess

  • 1Department of Chemistry, PO Box 30012, Texas A & M University, College Station, TX 77842-3012, USA.

Bioorganic & Medicinal Chemistry Letters
|July 23, 2003
PubMed

Abstract:

Phosphoramidite 1 was prepared, incorporated into oligonucleotides, and these were studied via thermal denaturation and circular dichroism.

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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