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Cooperative omega loops in cytochrome c: role in folding and function
Mallela M G Krishna1, Yan Lin, Jon N Rumbley
1Department of Biochemistry and Biophysics, Johnson Research Foundation, University of Pennsylvania School of Medicine, 422 Curie Blvd, 1007 Stellar Chance Bldg, Philadelphia, PA 19104-6059, USA. mmg@hx2.med.upenn.edu
Journal of Molecular Biology
|July 24, 2003
Abstract:
Hydrogen exchange experiments under slow exchange conditions show that an omega loop in cytochrome c (residues 40-57) acts as a cooperative unfolding/refolding unit under native conditions. This unit behavior accounts for an initial step on the unfolding pathway, a final step in refolding, and a number of other structural, functional and evolutionary properties.