Biooxidation of ferrous iron by immobilized Acidithiobacillus ferrooxidans in poly(vinyl alcohol) cryogel carriers
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Updated: Sep 21, 2026

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
Published on: April 22, 2016
Zhong-er Long1, Yunhong Huang, Zhaoling Cai
1State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, CAS, Beijing 100080, PR China. Longzhonger@163.com
Abstract:
PVA-cryogels entrapping about 10(9) cells of Acidithiobacillus ferrooxidans per ml of gel were prepared by freezing-thawing procedure, and the biooxidation of Fe2+ by immobilized cells was investigated in a 0.365 l packed-bed bioreactor. Fe2+ oxidation fits a plug-flow reaction model well. A maximum oxidation rate of 3.1 g Fe2+ l(-1) h(-1) was achieved at the dilution rate of 0.4 h(-1) or higher, while no obvious precipitate was determined at this time. In addition, cell-immobilized PVA-cryogels packed in bioreactor maintained their oxidative ability for more than two months under non-sterile conditions.
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