A biocompatible needle-type glucose sensor based on platinum-electroplated carbon electrode

Area of Science:

• Biomedical Engineering
• Biosensors
• Electrochemistry

Background:

• Accurate glucose monitoring is crucial for diabetes management.
• Existing glucose sensors face challenges in biocompatibility and long-term stability.
• Development of reliable and patient-friendly glucose sensing technologies is needed.

Purpose of the Study:

• To construct and evaluate a novel biocompatible needle-type glucose sensor.
• To assess the sensor's performance characteristics, including response time, reproducibility, stability, and dynamic range.
• To validate the sensor's accuracy for glucose determination in human serum samples.

Main Methods:

• Fabrication of a 3-electrode sensor using a platinum-electroplated carbon stick (working), Ag/AgCl (reference), and stainless steel needle (counter).
• Coating the working electrode with Nafion, immobilized glucose oxidase (GOD), and a diffusion-limiting biocompatible membrane.
• Performance evaluation through batch operation, stability tests in control serum, and comparison with a clinical autoanalyzer.

Main Results:

• The sensor exhibited a rapid response time (< 120 s) and excellent reproducibility (RE < 3%).
• Demonstrated good stability over 36 hours in control serum and a wide dynamic range (5-600 mg/dL).
• Achieved high correlation (R > 0.95) with a clinical autoanalyzer for serum glucose determination, indicating superior biocompatibility.

Conclusions:

• The developed needle-type glucose sensor is biocompatible and suitable for in vitro glucose monitoring.
• The sensor offers significant advantages in terms of speed, accuracy, stability, and ease of use.
• This technology holds promise for improving diabetes management through reliable glucose level assessment.