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Human papillomavirus type 45 propagation, infection, and neutralization
Margaret E McLaughlin-Drubin1, Susan Wilson, Brian Mullikin
1Department of Microbiology and Immunology, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA.
Virology
|August 2, 2003
Summary
Human papillomaviruses (HPVs) life cycle and virion morphogenesis were studied using organotypic culture. HPV45 virions were produced, infected keratinocytes, and showed cross-reactivity with HPV18 antisera.
Area of Science:
- Virology
- Cell Biology
- Dermatology
Background:
- Organotypic raft culture enables study of human papillomaviruses (HPVs) life cycle.
- Understanding HPV replication and virion assembly is crucial for vaccine development.
Purpose of the Study:
- To investigate the complete differentiation-dependent life cycle of HPV45 in organotypic culture.
- To analyze HPV45 virion morphogenesis and infectivity.
- To assess serological cross-reactivity between HPV45 and other HPV types.
Main Methods:
- Introduction of linearized HPV45 genomic DNA into primary keratinocytes.
- Epithelial stratification and differentiation in organotypic raft culture.
- Purification of HPV45 virions and in vitro infection assays.
- Neutralization assays using HPV VLP-specific antisera.
Main Results:
- HPV45 genomic DNA recircularized and maintained episomally in keratinocytes.
- Virion morphogenesis occurred following epithelial differentiation in raft culture.
- Purified HPV45 virions successfully infected keratinocytes in vitro.
- Infection neutralization demonstrated cross-reactivity between HPV45 and HPV18 VLP-specific antisera.
Conclusions:
- Organotypic culture is a viable model for studying HPV45 life cycle and virion production.
- HPV45 exhibits serological cross-reactivity with HPV18, suggesting potential for broader vaccine efficacy.