Related Experiment Videos
Detection of NO-induced DNA lesions by the modified aldehyde reactive probe (ARP) assay
Toshiaki Nakano1, Hiroaki Terato, Yoshihiro Yoshioka
1Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Higashi-Hiroshima 739-8526, Japan.
Nucleic Acids Research. Supplement (2001)
|August 9, 2003
Abstract:
When DNA is exposed to NO or HNO2, oxanine (Oxa) is formed as a major guanine lesion. For highly sensitive detection of Oxa using ARP, a probe molecule for DNA damage detection, the reactivity of ARP to Oxa was examined. Oxa site-specifically embedded in an oligonucleotide reacted with ARP but it took relatively long time (ca. 24 h) to completely convert Oxa to an ARP-labeled form.