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Development of an effective sample preparation method for the proteome analysis of body fluids using 2-D gel
Won-A Joo1, Do-Youn Lee, Chan-Wha Kim
1Graduate School of Life Sciences and Biotechnology, Korea University, Seoul.
Bioscience, Biotechnology, and Biochemistry
|August 13, 2003
Abstract:
A sample preparation is still the most critical step in two-dimensional electrophoresis (2-DE) and should be optimized for each type of sample. In this study, a protein extraction method from body fluids was developed using a combined centrifugal filter device and a sample treating buffer. When plasma, amniotic fluid, urine, and tear were tested with this method, the recovery of protein reached almost 90% and high-quality separation of 2-DE gel was obtained.