Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

TGFbeta1 signaling via alphaVbeta6 integrin.

Martin P Kracklauer1, Christian Schmidt, Guido M Sclabas

  • 1Section of Molecular Cell and Developmental Biology, Institute for Cellular and Molecular Biology, The University of Texas at Austin, 1 University Station, A4800, 78712, Austin, TX, USA. mordechai30@hotmail.com

Molecular Cancer
|August 26, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Preconception cardiometabolic markers and odds of developing adverse pregnancy outcomes: Results from the Hispanic Community Health Study/Study of Latinos (HCHS/SOL).

American journal of preventive cardiology·2026
Same author

Planetary Boundary for Novel Entities: Time for a Reboot.

Environmental science & technology·2026
Same author

A scalable, dividing cell model for the robust propagation and quantification of human sporadic Creutzfeldt-Jakob disease prions.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Aerobic Fitness Is the Main Determinant of Match-Related Physical Performance Decline in Professional Football Players (Part 2).

Journal of strength and conditioning research·2026
Same author

Availability of mechanical circulatory support (MCS) and hospital survival in ST-segment elevation myocardial infarction related cardiogenic shock (STEMI-CS).

European heart journal. Acute cardiovascular care·2026
Same author

Venetoclax and hypomethylating agents synergize to increase cell death and metabolic remodeling in acute B-lymphoblastic leukemia cells.

Molecular metabolism·2026

Transforming growth factor beta1 (TGFbeta1) signals through alphaVbeta6 integrin, independent of the SMAD pathway. This alternate pathway inhibits carcinoma cell growth, even in SMAD4-deficient cells, revealing a new target for cancer therapy.

Area of Science:

  • Cell Biology
  • Molecular Oncology
  • Cancer Signaling

Background:

  • Transforming growth factor beta1 (TGFbeta1) inhibits epithelial cell growth and maintains tissue homeostasis.
  • Carcinoma cells often show reduced sensitivity to TGFbeta1, implicating it in cancer development.
  • The tumor suppressor gene DPC4/SMAD4 is crucial for TGFbeta1 growth inhibition, but SMAD4-independent pathways exist.

Purpose of the Study:

  • To investigate the SMAD4-independent TGFbeta1 signaling pathway in carcinoma cells.
  • To identify novel ligands and mediators of TGFbeta1-induced growth inhibition.

Main Methods:

  • Investigated TGFbeta1 binding to integrins.
  • Analyzed signaling protein activation in response to TGFbeta1.
  • Assessed the role of alphaVbeta6 integrin in TGFbeta1 signaling.

Related Experiment Videos

  • Measured cell cycle inhibitor expression (p21/WAF1, p27) and cell growth.

    • Main Results:

    • Mature TGFbeta1 directly binds to integrin alphaVbeta6, independent of the RGD motif.
    • TGFbeta1-alphaVbeta6 interaction activates signaling proteins in TGFbeta1-sensitive carcinoma cells.
    • This interaction upregulates cell cycle inhibitors p21/WAF1 and p27, inhibiting growth in both SMAD4-deleted and SMAD4-wildtype cells.

    Conclusions:

    • A SMAD4-independent TGFbeta1 signaling pathway involving alphaVbeta6 integrin exists.
    • This pathway utilizes the Ras/MAP kinase pathway and does not require an RGD motif.
    • Both SMAD-dependent and alphaVbeta6-mediated pathways are necessary for a complete TGFbeta1 signal.