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A simple enzymatic quantitative analysis of triglycerides in tissues
H Danno1, Y Jincho, S Budiyanto
1Department of Food Chemistry, Faculty of Agriculture, Tohoku University, Sendai, Japan.
Journal of Nutritional Science and Vitaminology
|October 1, 1992
Summary
Researchers developed a novel method to quantify tissue triglyceride levels using a modified enzymatic kit. This technique accurately measures triglyceride concentrations in tissue samples, offering a valuable tool for biological research.
Area of Science:
- Biochemistry
- Analytical Chemistry
Background:
- Triglyceride quantification is essential for understanding lipid metabolism.
- Existing enzymatic kits are primarily optimized for plasma, limiting their application in tissue analysis.
Purpose of the Study:
- To adapt a commercially available enzymatic kit for accurate triglyceride measurement in tissue samples.
- To validate a modified protocol for tissue triglyceride determination.
Main Methods:
- Tissue lipid extracts were dissolved in alcohol.
- A mixture of tert-butyl alcohol and Triton X-100/methyl alcohol was added to the dissolved lipids.
- The modified solution was analyzed using the enzymatic kit and a spectrophotometer.
Main Results:
- The modified method successfully quantified triglyceride levels in tissues.
- The presence of organic surfactants (tert-butyl alcohol, methyl alcohol) and Triton X-100 did not interfere with enzymatic activity.
- The method allowed for triglyceride level determination in the range of 10 to 90 nmol.
Conclusions:
- A reliable method for measuring tissue triglyceride levels using a modified enzymatic kit was established.
- This adaptation expands the utility of enzymatic kits for broader lipid analysis applications.
- The method provides accurate quantification of tissue triglycerides without interference from common surfactants.