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[Cloning and sequence analysis of the molecular-adjuvant gene of the complement C3d of mouse]
Meiqin Sun1, Liansan Zhao, Shouchun Chen
1Department of Infecious Diseases, West China Hospital, Sichuan University, Chengdu 610041, China.
Objective:
Considering that the complement C3d plays a stronger positive role in the control of immunologic system, we constructed the recombinant plasmid of the molecular-adjuvant gene of the complement C3d in order to enhance the immunologic efficacy of the gene vaccine.
Methods:
The main points were amplifying the segment of complement C3d gene by using of PCR; inserting it into PGEM-T; detecting it by digesting with Eco RI and sequencing; the whole length being 897 bp; then sub-cloning it into pVAX1.
Results:
The DNA sequencing confirmed the C3d sequence.
Conclusion:
We have successfully cloned the gene of C3d and constructed the recombinant plasmid of pVAX1 C3d.
