Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sputum induction leads to a decrease of exhaled nitric oxide unrelated to airflow.

J Beier1, K M Beeh, O Kornmann

  • 1Pulmonary Dept, Internal Medicine, University Hospital, Mainz, Germany. j.beier@3-med.klinik.uni-mainz.de

The European Respiratory Journal
|September 4, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Histological evaluation of cardiac remodelling in equine athletes.

Scientific reports·2024
Same author

Adherence to inhaled corticosteroids and long-acting β2-agonists in asthma: A MASK-air study.

Pulmonology·2023
Same author

Rhinitis associated with asthma is distinct from rhinitis alone: The ARIA-MeDALL hypothesis.

Allergy·2023
Same author

Identification by cluster analysis of patients with asthma and nasal symptoms using the MASK-air® mHealth app.

Pulmonology·2022
Same author

Reduction of SARS-CoV-2 viral load in exhaled air by antiseptic chewing gum: a pilot trial.

Infection·2022
Same author

Bronchodilator Reversibility in the GAN Severe Asthma Cohort.

Journal of investigational allergology & clinical immunology·2022
Same journal

Beyond the 5th percentile: rethinking diagnostic thresholds in pulmonary function testing.

The European respiratory journal·2026
Same journal

A Transcriptomic Atlas of Chronic Lung Allograft Dysfunction.

The European respiratory journal·2026
Same journal

Risk of cancer and mortality in patients with interstitial lung diseases: Danish cohort study.

The European respiratory journal·2026
Same journal

Proposing a minimal important difference in NTproBNP in pulmonary arterial hypertension.

The European respiratory journal·2026
Same journal

Unlocking growth potential: Ivacaftor therapy and developmental gains in lung and height in a cohort study of children and young adults with cystic fibrosis.

The European respiratory journal·2026
Same journal

Decoding the Lymphangioleiomyomatosis (LAM) Niche Microenvironment <i>via</i> Integrative Analysis of Single Cell Multiomics and Spatial Transcriptomics.

The European respiratory journal·2026
See all related articles

Sputum induction with hypertonic saline significantly reduces exhaled nitric oxide (eNO) levels in adults with asthma or atopy. This effect is reproducible and lasts for hours, impacting eNO measurements.

Area of Science:

  • Pulmonology
  • Allergy and Immunology
  • Biochemistry

Background:

  • Exhaled nitric oxide (eNO) and induced sputum are key noninvasive methods for assessing airway inflammation in asthma.
  • These methods are frequently used together, raising questions about potential interactions.

Purpose of the Study:

  • To investigate the impact of sputum induction on eNO levels in adults.
  • To examine the time course of eNO production following sputum induction in relation to lung function (FEV1).

Main Methods:

  • Measurements of eNO and FEV1 were taken before and after sputum induction with hypertonic saline (4%) in 41 adult patients.
  • A control group (13 subjects) underwent induction with isotonic saline.
  • Repeatability and 24-hour kinetics of eNO changes were also assessed.

Related Experiment Videos

Main Results:

  • Hypertonic saline, but not isotonic saline, caused a significant, reproducible decrease in eNO levels immediately post-induction.
  • This eNO reduction was independent of changes in FEV1.
  • eNO levels remained decreased for up to 4 hours and returned to baseline within 24 hours.

Conclusions:

  • Sputum induction using hypertonic saline induces a temporary but significant reduction in exhaled nitric oxide in adults with atopy or mild asthma.
  • This methodological interference must be considered when interpreting sequential eNO and sputum induction results.