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StCDPK1 is expressed in potato stolon tips and is induced by high sucrose concentration
Marcela Raíces1, Rita M Ulloa, Gustavo C MacIntosh
1Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, CONICET, FCEyN, Universidad de Buenos Aires. Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina.
Abstract:
StCDPK1 encodes a calcium-dependent protein kinase (CDPK) from Solanum tuberosum, which is transiently induced upon tuberization in swelling stolons. In situ hybridization determined that StCDPK1 mRNA is localized in the apical dome of tuberizing stolon tips, close to the region where sucrose was reported to accumulate. The expression of StCDPK1, and other tuber-specific genes was enhanced when in vitro-cultured potato plants were transferred to high sucrose or high sorbitol containing media. Glucose, fructose or a mixture of both showed no effect on CDPK expression. Okadaic acid blocked sucrose-inducible gene expression, suggesting that phosphatases from the PP1/PP2A family could also participate in the regulation of StCDPK1 and other tuberization-related genes.