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Hydrogen peroxide-induced DNA damages in vitro revealed by electron microscopy
S Chaudhuri1, N P Bhattacharyya, S B Bhattacharjee
1Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, Calcutta, India.
Journal of Electron Microscopy
|February 1, 1992
Abstract:
A plasmid pSV2neo (5.6 kb) which is a recombinant SV40-based vector was subjected to hydrogen peroxide treatment in presence of iron in vitro (Fenton reaction). Transmission electron microscopy revealed that H2O2 through the Fenton reaction introduced different types of damages in DNA. Besides the expected nicked circular and linear molecules, single-stranded loops and plasmid multimers could be characterized. These observations on DNA damages might be helpful for better understanding of the effect of hydrogen peroxide on living cells in vivo.