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Site-specific incorporation of [125I]iododeoxyuridine into DNA
N Scherberg1, I Bloch, P Gardner
1University of Chicago Hospitals and Clinics, IL.
International Journal of Radiation Applications and Instrumentation. Part A, Applied Radiation and Isotopes
|July 1, 1992
Abstract:
A procedure for the incorporation of [125I]IdU into specific sites in DNA is described. The approach depends upon attachment of radioiododeoxyuridine to a controlled pore glass support which is then used for automated synthesis of an oligomer. The resulting oligomer, containing a terminal 3'[125I]iododeoxyuridine, is used as a primer during DNA synthesis catalyzed by the Taq polymerase employing thermal cycling. The product formed includes the radioiodonucleotide at a single internal site determined by the length of the oligomer.