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Improved enzyme immunosorbent assay for mouse prolactin using penicillinase as label
Y Tomita1, R W Engelman, I Bauer-Sardina
1Department of Pediatrics/St. Petersburg, University of South Florida 33701-4899.
Journal of Immunological Methods
|July 6, 1992
Summary
A new enzyme immunosorbent assay (EIA) accurately measures mouse prolactin. This sensitive and reproducible method offers results comparable to radioimmunoassay (RIA), providing a reliable tool for research.
Area of Science:
- Endocrinology
- Immunology
- Biochemistry
Background:
- Prolactin is a crucial hormone regulating reproduction and other physiological processes.
- Accurate measurement of mouse prolactin is essential for reproductive and endocrine research.
Purpose of the Study:
- To establish a sensitive, rapid, and reproducible enzyme immunosorbent assay (EIA) for quantifying mouse prolactin.
- To validate the performance of the developed mouse prolactin EIA against established radioimmunoassay (RIA) methods.
Main Methods:
- Modified a human prolactin EIA method using penicillinase as the labeling enzyme.
- Employed rabbit anti-mouse prolactin antibody and goat anti-rabbit Ig antibody.
- Quantified prolactin by measuring absorbance at 620 nm using an ELISA reader.
Main Results:
- The developed EIA demonstrated high sensitivity (1.7 ng/ml), comparable to RIA.
- Prolactin levels measured by EIA showed excellent correlation with RIA (r = 0.943).
- Intra-assay and inter-assay coefficients of variation (CVs) for EIA were comparable to RIA.
Conclusions:
- The established EIA is a simple, sensitive, rapid, and reproducible method for mouse prolactin measurement.
- This EIA serves as a viable alternative to RIA for quantifying mouse prolactin in serum samples.
- The assay provides reliable and accurate results, supporting its use in various research applications.