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Dipeptidylpeptidase IV in human leukemic B- and T-cells
V S Bylinkina1, L A Lokshina, R S Samojlova
1Institute of Biological and Medical Chemistry, Russian Academy of Medical Sciences, Moscow.
Biochemistry International
|October 1, 1992
Summary
Dipeptidylpeptidase IV (DPP-IV) is present in leukemic B- and T-cells. High DPP-IV activity was found in early T-cells and some activated B-cells, challenging its role as a T-cell marker.
Area of Science:
- Immunology
- Biochemistry
- Hematology
Background:
- Dipeptidylpeptidase IV (DPP-IV) is an enzyme found on cell surfaces.
- Its role in lymphoproliferative diseases is not fully understood.
- DPP-IV has been considered a marker for activated T-cells.
Purpose of the Study:
- To investigate DPP-IV activity in lymphoid cells from patients with lymphoproliferative disorders.
- To determine if DPP-IV is exclusively associated with T-cell activation.
Main Methods:
- Enzyme activity assays on leukemic T- and B-cell lysates.
- Phenotypic analysis using cell surface markers (CD 25, CD 30, CD 11c).
- Investigation of DPP-IV localization on intact leukemic cells.
Main Results:
- DPP-IV activity was detected in all studied leukemic T- and B-cells.
- Elevated DPP-IV levels were observed in early T-cells and activated T-lymphocytes.
- Significant DPP-IV activity was also found in activated leukemic B-cells, particularly those expressing CD 25 and CD 11c.
Conclusions:
- DPP-IV is present in the plasma membrane of both leukemic B- and T-cells.
- DPP-IV is not exclusive to activated T-cells and is also found in early T-cells and certain activated B-cells.
- These findings broaden the understanding of DPP-IV expression in lymphoproliferative diseases.