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Related Experiment Videos

Fluoride absorption from the rat urinary bladder: a pH-dependent event.

G M Whitford, D H Pashley, K E Reynolds

    The American Journal of Physiology
    |January 1, 1977
    PubMed
    Summary

    Bladder fluoride absorption significantly decreases as pH increases from 1.85 to 5.50. This finding suggests hydrogen fluoride diffusion is the primary mechanism for fluoride transport in the bladder.

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    Fluoride and Oral Health.

    Community dental health·2016

    Area of Science:

    • Physiology
    • Toxicology
    • Biochemistry

    Background:

    • Fluoride absorption is crucial for understanding fluoride toxicity and therapeutic applications.
    • The influence of urinary pH on fluoride transport remains incompletely understood.
    • Investigating pH gradients is essential for elucidating fluoride's pharmacokinetic behavior.

    Purpose of the Study:

    • To investigate the effect of varying pH levels on urinary bladder absorption of fluoride.
    • To determine the relationship between pH gradients and fluoride transport dynamics.
    • To identify the mechanism of fluoride absorption in the bladder.

    Main Methods:

    • Anesthetized rats were used to study urinary bladder absorption of stable and radiofluoride.
    • Experiments were conducted across a pH range of 1.85 to 7.90 and fluoride concentrations from 0.012 to 8.81 mM.
    • [14C]inulin was employed as a marker for water migration and dilution effects.

    Main Results:

    • Fluoride absorption demonstrated an inverse relationship with pH between 1.85 and 5.50.
    • Mean radiofluoride absorption decreased from 70% at pH 1.85 to 5% at pH 5.50.
    • Absorption above pH 5.50 was minimal and pH-independent, unaffected by fluoride concentration, buffers, or urine presence.

    Conclusions:

    • Bladder fluoride absorption is significantly pH-dependent, decreasing markedly as pH rises.
    • The findings support a first-order absorptive process driven by the nonionic diffusion of hydrogen fluoride.
    • Understanding pH-dependent absorption is critical for managing fluoride exposure and treatment.

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