Related Experiment Video
Updated: Jul 10, 2026
10:58
Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes
Published on: June 28, 2012
Studies on hybridoma and insect cells in suspension culture
1Department of Chemical Engineering, University of Queensland.
Australian Journal of Biotechnology
|April 1, 1990
Abstract:
Our laboratory has over a period of four years conducted extensive research into the metabolism and batch, fed-batch, continuous, perfusion and serum-free culture of hybridomas with a view to increasing the productivity of these animal cell lines. A brief overview of this work and future plans will be presented here. In addition an overview of our more limited work on the Baculovirus expression system using insect cell culture, begun during 1989, will also be given.
Related Concept Videos
01:21Cell Culture
Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media
01:31Hybridoma Technology
Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...