Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Optimization and simulation of continuous affinity-recycle extraction (care).

N F Gordon1, H Tsujimura, C L Cooney

  • 1Department of Chemical Engineering and Biotechnology Process Engineering Centers, Massachusetts Institute of Technology, Cambridge 02139.

Bioseparation
|January 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Leveraging Industry-Academia Collaborations in Adaptive Biomedical Innovation.

Clinical pharmacology and therapeutics·2016
Same author

Exercise Testing Update.

The Physician and sportsmedicine·2016
Same author

Studies on the overproduction of indole-containing metabolites by a methanol-utilizing yeast : Hansenula polymorpha.

Applied biochemistry and biotechnology·2013
Same author

Are we prepared for animal cell technology in the 21st century?

Cytotechnology·2012
Same author

Multiple lymphomatous polyposis overexpressing cyclin D1.

International journal of clinical oncology·2010
Same author

Innovative approaches to comprehensive cardiovascular disease risk reduction in clinical and community-based settings.

Current atherosclerosis reports·2001
Same journal

Study of the factors influencing peak asymmetry on chromatography using a molecularly imprinted polymer prepared by the epitope approach.

Bioseparation·2003
Same journal

Molecularly imprinted polymer as storage medium for an analyte.

Bioseparation·2003
Same journal

A molecular imprinted polymer with recognition properties towards the carcinogenic mycotoxin ochratoxin A.

Bioseparation·2003
Same journal

Molecular imprinting and solid phase extraction of flavonoid compounds.

Bioseparation·2003
Same journal

MIP-ligand binding assays (pseudo-immunoassays).

Bioseparation·2003
Same journal

Selective solid-phase extraction of bio- and environmental samples using molecularly imprinted polymers.

Bioseparation·2003
See all related articles

Continuous affinity recycle extraction (CARE) offers an efficient method for protein purification. This technique uses well-mixed reactors and recirculating adsorbents for improved recovery and concentration, outperforming traditional column chromatography.

Area of Science:

  • Biochemical Engineering
  • Separation Science
  • Downstream Processing

Background:

  • Protein purification is critical in biotechnology and pharmaceuticals.
  • Conventional methods like column chromatography can be inefficient and costly.
  • Continuous processing offers potential advantages in efficiency and scalability.

Purpose of the Study:

  • To describe the simulation and optimization of Continuous Affinity Recycle Extraction (CARE).
  • To establish the feasibility of CARE for enzyme recovery and isolation.
  • To compare CARE performance with conventional column chromatography.

Main Methods:

  • Solid/liquid contact in well-mixed reactors with recirculating adsorbent particles.
  • Development and application of a mathematical model for system optimization.

Related Experiment Videos

  • Experimental validation of design modifications, objective functions, and start-up protocols.

    • Main Results:

    • Demonstrated successful recovery and isolation of beta-galactosidase using CARE.
    • Optimized CARE design (e.g., adding wash stages, multiple adsorption stages) enhanced purification, concentration, and recovery.
    • Developed and validated optimal start-up protocols to minimize steady-state time.
    • Showcased the benefits of early integration of CARE in downstream processing for simultaneous clarification, concentration, and purification.

    Conclusions:

    • CARE is a feasible and effective protein purification technique.
    • Optimization of CARE design and operation significantly improves performance.
    • Early implementation of CARE in downstream processing yields substantial benefits.
    • CARE demonstrates superior or comparable purification performance to conventional column chromatography.