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Extraction of proteins from animal tissue using multiphase aqueous systems
1Biochemical Processing Centre, Department of Scientific and Industrial Research, Palmerston North, New Zealand.
Bioseparation
|January 1, 1990
Summary
Animal tissue remains a key source for enzymes and protein hormones. Aqueous phase systems offer promising advantages for protein purification from tissues, demonstrated at lab and pilot scales.
Area of Science:
- Biotechnology
- Biochemistry
- Protein purification
Background:
- Animal tissues are a significant source of valuable enzymes and protein hormones.
- Traditional methods for protein extraction can be inefficient or costly.
- Aqueous phase systems present an alternative for protein purification.
Purpose of the Study:
- To review the potential of aqueous phase systems for protein purification from animal tissues.
- To discuss the process and economic aspects of these systems.
- To highlight the advantages of aqueous phase systems in protein extraction.
Main Methods:
- Review of existing literature on aqueous phase systems for protein purification.
- Analysis of simple phase systems and affinity partitioning systems.
- Evaluation of laboratory and pilot scale demonstrations.
Main Results:
- Aqueous phase systems demonstrate considerable potential for extractive protein purification.
- Laboratory scale success for various enzymes and pilot scale success for some proteins have been shown.
- Both simple and affinity partitioning systems are effective.
Conclusions:
- Aqueous phase systems are a viable and advantageous method for purifying proteins from animal tissues.
- Further development could lead to industrial-scale applications.
- These systems offer specific benefits over conventional methods.