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Isolation and characterization of the alkaline protease gene of Aspergillus oryzae
K Murakami1, Y Ishida, A Masaki
1Research and Development Division, Green Cross Corporation, Osaka, Japan.
Agricultural and Biological Chemistry
|November 1, 1991
Abstract:
The genomic DNA for the alkaline protease (Alp) of the fungus Aspergillus oryzae was isolated using synthetic oligonucleotides as hydridization probes, and the complete nucleotide sequence was identified. The Alp gene is 1374 nucleotides long and contains three introns, one of which is in the pro region and two in the mature coding region. Sequences related to the TATA box (TATAAAT) and the CAAT box (CCAAAT) were found in the 5'-noncoding region. Primer extension analysis showed that three transcriptional start points are present.