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Related Experiment Videos

Protein isolation by solution-controlled gel sorption.

S H Gehrke1, J Robeson, J F Johnson

  • 1Department of Chemical Engineering, University of Cincinnati, Ohio 45221-0171.

Biotechnology Progress
|July 1, 1991
PubMed
Summary
This summary is machine-generated.

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This study introduces a novel polymer gel method for protein isolation, simplifying separation and recycling compared to traditional aqueous extraction. The technique effectively isolates various proteins using poly(ethylene glycol) and dextran gels.

Area of Science:

  • Biochemistry
  • Polymer Science
  • Separation Science

Background:

  • Conventional two-phase aqueous extraction is a common method for protein separation.
  • Existing methods can present challenges in protein recovery and material recycling.

Purpose of the Study:

  • To illustrate principles for isolating proteins using a polymer gel phase.
  • To demonstrate a simpler alternative to conventional aqueous extraction for protein purification.

Main Methods:

  • Proteins were isolated by sorption into a polymer gel phase (poly(ethylene glycol) and dextran).
  • Separation was driven by the addition of water-soluble polymer to the protein solution.
  • The effect of polymer molecular weight and concentration on protein sorption was investigated.

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Main Results:

  • The polymer gel method proved analogous to two-phase aqueous extraction but offered simpler protein and polymer separation.
  • Increasing poly(ethylene glycol) molecular weight and concentration enhanced the sorption of ovalbumin, bovine serum albumin, cytochrome c, and hemoglobin.
  • Quantitative protein recovery was achieved by immersing the gel in a polymer-free solution.

Conclusions:

  • Polymer gel sorption presents a simplified and efficient approach for protein isolation.
  • This method facilitates easier separation of proteins from polymers and allows for gel phase recycling.
  • The technique is effective for purifying various proteins, with sorption influenced by polymer characteristics.