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Structures of the O chains from lipopolysaccharides of Campylobacter jejuni serotypes O:23 and O:36
G O Aspinall1, A G McDonald, H Pang
1Department of Chemistry, York University, Toronto, Ontario, Canada.
Abstract:
Lipopolysaccharides of C. jejuni serotypes O:23 and O:36 have been shown to contain structurally variable O polysaccharide chains with repeating units of four closely-related types: ----3)-beta-D-GlcpNAc-(1----3)-alpha-D-Galp-(1----2)-6d-alpha-D-alt-H epp-(1---- , ----3)-beta-D-GlcpNAc-(1----3)-alpha-D-Galp-(1----2)-6d-3-Me-alpha-D-alt -Hepp- (1----, ----3)-beta-D-GlcpNAc-(1----3)-alpha-D-Galp-(1----2)-D-glycero-alpha-D-a lt-Hepp - (1----, and ----3)-beta-D-GlcpNAc-(1----3)-alpha-D-Galp-(1----2)-3-Me-D-glycero-alph a-D-alt - Hepp-(1----. Structural methods included 1H- and 13C-NMR spectroscopy, methylation linkage analysis, fast atom bombardment mass spectrometry of methylated glycans, and selective fragmentations by the Smith degradation and N-deacetylation-nitrous acid deamination.
