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Related Experiment Videos

Two-site direct immunoassay specific for active renin.

D Simon1, D J Hartmann, G Badouaille

  • 1Sanofi Recherche, Montpellier, France.

Clinical Chemistry
|October 1, 1992
PubMed
Summary

A new assay accurately measures active human renin using monoclonal antibodies and magnetic beads. This sensitive method distinguishes active from inactive renin, crucial for understanding renin

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Area of Science:

  • Biochemistry
  • Immunology
  • Assay Development

Background:

  • Active human renin is a key enzyme in the renin-angiotensin-aldosterone system.
  • Measuring active renin specifically is crucial for diagnosing and managing various cardiovascular and renal conditions.
  • Existing assays may lack specificity or sensitivity for active renin.

Purpose of the Study:

  • To develop a highly sensitive and specific immunoradiometric assay for active human renin.
  • To characterize the performance of the newly developed assay.
  • To measure active plasma renin concentrations in normotensive subjects.

Main Methods:

  • Development of a two-step immunoradiometric assay using two monoclonal antibodies (MAbs).
  • One MAb coupled to magnetic beads (Magnogel) for capture.

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  • Second MAb directed against active renin, radiolabeled with 125I for detection.
  • Assay specificity confirmed by measuring active renin in the presence of inactive renin.

    • Main Results:

    • The assay is specific for active human renin, distinguishing it from inactive forms.
    • High sensitivity achieved, detecting as little as 0.9 pg of renin per tube (3.5 ng/L).
    • Low intra- and interassay coefficients of variation (< 10%) indicate good reproducibility.
    • Active plasma renin concentrations in normotensive subjects ranged from 7 to 40 ng/L.

    Conclusions:

    • A novel, sensitive, and specific immunoradiometric assay for active human renin has been successfully developed.
    • This assay provides a reliable tool for quantifying active renin without enzymatic steps.
    • The findings establish reference ranges for active plasma renin in normotensive individuals.