Genetically engineered rice resistant to rice stripe virus, an insect-transmitted virus
1Plantech Research Institute, Yokohama, Japan.
Summary
Transgenic rice plants expressing the rice stripe virus coat protein (CP) gene showed significant resistance to infection. This resistance was stably inherited by progeny, demonstrating CP-mediated viral protection in cereals.
Area of Science:
- Agricultural Science
- Plant Biotechnology
- Virology
Background:
- Rice stripe virus (RSV) poses a significant threat to rice production worldwide.
- Developing resistant crop varieties is crucial for sustainable agriculture.
Purpose of the Study:
- To investigate the efficacy of coat protein (CP) gene-mediated resistance against RSV in japonica rice varieties.
- To assess the stability and heritability of this resistance trait.
Main Methods:
- Introduction of the RSV CP gene into rice protoplasts via electroporation.
- Generation and analysis of transgenic rice plants for CP expression and viral resistance.
- Evaluation of resistance in selfed progeny of primary transformants.
Main Results:
- Transgenic rice plants achieved high levels of CP expression (up to 0.5% of total soluble protein).
- Significant resistance to RSV infection was observed in primary transformants and their progeny.
- Absence of RSV-specific disease protein in transgenic plants 8 weeks post-inoculation.
Conclusions:
- CP-mediated resistance is effective in japonica rice against RSV.
- The CP gene and viral resistance are stably transmitted to subsequent generations.
- This approach offers a viable strategy for engineering resistance in cereals against insect-borne viruses.
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