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DISINFECTION OF AEROSOLIZED PATHOGENIC FUNGI ON LABORATORY SURFACES. II. CULTURE PHASE.

R H KRUSE, T D GREEN, R C CHAMBERS

    Applied Microbiology
    |March 1, 1964
    PubMed
    Summary
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    Fungicides are less effective against the spore phase of Blastomyces dermatitidis, Coccidioides immitis, and Histoplasma capsulatum. Adding a wetting agent improved fungicide efficacy for surface disinfection.

    Area of Science:

    • Mycology
    • Medical Mycology
    • Fungicide Efficacy

    Background:

    • Blastomyces dermatitidis, Coccidioides immitis, and Histoplasma capsulatum are pathogenic fungi.
    • Laboratory surfaces can become contaminated with the aerosolized spore phase of these fungi.
    • Effective disinfection protocols are crucial for preventing fungal transmission.

    Purpose of the Study:

    • To evaluate the efficacy of various fungicides against the spore phase of three dimorphic fungi.
    • To compare the susceptibility of the spore (culture) phase versus the yeast (tissue) phase to fungicides.
    • To determine the impact of wetting agents on fungicide effectiveness.

    Main Methods:

    • Laboratory surfaces were intentionally contaminated with aerosolized spores of Blastomyces dermatitidis, Coccidioides immitis, and Histoplasma capsulatum.
    Keywords:
    ACETATESALCOHOL, ETHYLANTIFUNGAL AGENTSBLASTOMYCESCOCCIDIOIDESEXPERIMENTAL LAB STUDYFORMALDEHYDEHISTOPLASMAPHENOLSSTERILIZATION

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  • The fungicidal activity of several chemical agents was tested against the contaminated surfaces.
  • Experiments were conducted with and without the addition of a wetting agent to assess its influence.

    • Main Results:

    • The spore (culture) phase of the fungi demonstrated greater resistance to fungicides compared to the yeast (tissue) phase.
    • The inclusion of a wetting agent significantly enhanced the disinfecting efficiency of several tested fungicides.
    • Disinfection time and concentration parameters could be extrapolated from plotted data.

    Conclusions:

    • Standard fungicides exhibit reduced efficacy against the environmentally relevant spore form of these pathogenic fungi.
    • Wetting agents are a valuable addition to fungicide formulations for improving surface disinfection.
    • Quantitative data allows for the prediction of effective disinfection strategies based on time and concentration.