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Recombinant Retroviral Production and Infection of B Cells
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Published on: February 18, 2011

RECOMBINATION OF ANTIBODY POLYPEPTIDE CHAINS IN THE PRESENCE OF ANTIGEN

H METZGER, M MANNIK

    The Journal of Experimental Medicine
    |November 1, 1964
    PubMed

    Conditions were developed by which the separated H and L chains of gamma(2) globulins recombined to form four-chained molecules in good yields. In the absence of antigen, anti-2,4-dinitrophenyl (anti-DNP) H chains randomly reassociated with a mixture of antibody and non-specific gamma(2) globulin L chains. In the presence of a specific hapten, however, the antibody H chains preferentially interacted with the anti-DNP L chains. Antibody H chain-antibody L chain recombinants formed in the presence of hapten were more active than the corresponding recombinants formed in the absence of hapten. Speculations are made regarding the possible mechanisms and biological significance of these effects.

    Keywords:
    AMINOCAPROIC ACIDANTIBODIESANTIGEN-ANTIBODY REACTIONSDIALYSISDINITROPHENOLSEXPERIMENTAL LAB STUDYGAMMA GLOBULINHAPTENSIMMUNOCHEMISTRYIODINE ISOTOPESPEPTIDES

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