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[Effect of peptidoglycan preparations on phage inactivation: decrease in phage 187 binding using monosaccharides].

U Beige, G Seltmann

    Zhurnal Mikrobiologii, Epidemiologii I Immunobiologii
    |June 1, 1977
    PubMed
    Summary
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    Monosaccharides, particularly galactosamine and glucosamine, inhibit phage 187 binding to peptidoglycan. Calcium ions enhance this effect for specific monosaccharides, impacting phage adsorption to Staphylococcus aureus.

    Area of Science:

    • Microbiology
    • Biochemistry
    • Molecular Biology

    Background:

    • Bacteriophages are viruses that infect bacteria.
    • Phage-host interactions are crucial for phage therapy and understanding bacterial resistance.
    • Peptidoglycan is a major component of bacterial cell walls and a target for phage binding.

    Purpose of the Study:

    • To investigate the effect of monosaccharides on the binding of phage 187 to peptidoglycan.
    • To determine the role of calcium ions in modulating monosaccharide-mediated inhibition of phage binding.
    • To assess the impact of monosaccharides on phage adsorption to Staphylococcus aureus cells.

    Main Methods:

    • In vitro binding assays using purified peptidoglycan and phage 187.
    • Testing various monosaccharides (galactosamine hydrochloride, glucosamine hydrochloride, N-acetylgalactosamine, N-acetylglucosamine, glucose, galactose, mannose).

    Related Experiment Videos

  • Assessing the influence of calcium ions (Ca2+) on binding inhibition.
  • Phage adsorption experiments with Staphylococcus aureus cells in the presence and absence of Ca2+.

    • Main Results:

    • Monosaccharides decreased phage 187 binding to peptidoglycan, with galactosamine hydrochloride and glucosamine hydrochloride being most effective.
    • N-acetylgalactosamine, N-acetylglucosamine, glucose, and galactose showed moderate to weak inhibitory effects, while mannose had minimal impact.
    • Calcium ions enhanced the inhibitory effect of N-acetylgalactosamine and N-acetylglucosamine on phage-peptidoglycan binding.
    • These monosaccharides reduced phage 187 adsorption to Staphylococcus aureus cells, but could not release irreversibly bound phage without Ca2+.

    Conclusions:

    • Specific monosaccharides can interfere with phage-bacterial cell wall interactions.
    • Calcium ions play a role in enhancing the inhibitory potential of certain monosaccharides against phage binding.
    • Monosaccharides show potential as adjuncts to modulate phage-host interactions in Staphylococcus aureus infections.