Related Experiment Video

Updated: Sep 2, 2026

Investigating Flagella-Driven Motility in Escherichia coli by Applying Three Established Techniques in a Series
07:59

Investigating Flagella-Driven Motility in Escherichia coli by Applying Three Established Techniques in a Series

Published on: May 10, 2020

ISOLATION OF SALMONELLAE BY SELECTIVE MOTILITY SYSTEMS

P F STUART, H PIVNICK

    Applied Microbiology
    |May 1, 1965
    PubMed

    Insights

    This study introduces a selective migration (SM) method using U-tubes for isolating Salmonella bacteria from swine and human feces. The SM technique is efficient, requiring less media and time while achieving equal or superior recovery rates compared to standard methods.

    Area of Science:

    • Microbiology
    • Veterinary Medicine
    • Public Health

    Background:

    • Salmonella infections pose significant risks to both animal and human health.
    • Accurate and efficient isolation of Salmonella is crucial for diagnosis and control.

    Purpose of the Study:

    • To evaluate a novel selective migration (SM) method for Salmonella isolation from fecal samples.
    • To compare the efficacy of the SM method against standard diagnostic procedures.

    Main Methods:

    • Utilized modified "U" tubes containing semisolid enrichment (SM) media for selective migration of Salmonella.
    • Compared Salmonella recovery rates between the SM technique and standard laboratory methods using swine and human fecal samples.

    Main Results:

    • The SM method demonstrated equal or superior Salmonella recovery compared to standard diagnostic techniques.
    • Primary Salmonella isolates obtained via SM enrichment were notably pure, with reduced contamination from other fecal bacteria.
    • The SM method required significantly less media (15%) and reduced time and skill.

    Conclusions:

    • The selective migration (SM) method offers a more efficient and effective approach for Salmonella isolation from fecal matter.
    • This technique has the potential to improve diagnostic laboratory workflows for Salmonella detection.
    Keywords:
    CULTURE MEDIAEQUIPMENT AND SUPPLIESFECESSALMONELLASALMONELLA PARATYPHISALMONELLA TYPHIMURIUMSALMONELLA TYPHOSASWINE

    More Related Videos

    Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages10:33

    Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages

    Published on: October 25, 2017

    Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples06:12

    Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples

    Published on: October 25, 2018

    Related Concept Videos

    Overview Of Cell Separation And Isolation01:20

    Overview Of Cell Separation And Isolation

    Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.