Related Experiment Videos
Analysis of processing-defective variants of human tRNA(Val)
Yoshio Kato1, Masayuki Sano, Kazunari Taira
1Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Hongo, Tokyo 113-8656, Japan.
Nucleic Acids Research. Supplement (2001)
|September 27, 2003
Abstract:
Eukaryotic tRNAs are transcribed, processed and exported from the nucleus to the cytoplasm to participate in protein biosynthesis. A variant of human gene for tRNA(Val), HtV4, is not processed neither its 5'- nor its 3'-flanking sequences. By RT-PCR analysis and in situ hybridization, we demonstrated that the HtV4 was constitutively expressed without processing, and localized in the nucleus in HeLa cells.