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Encapsulation of DNA with neutral glycocluster nanoparticles. A step toward artificial viruses
Shinsuke Sando1, Toshinori Sasaki, Yasuhiro Aoyama
1Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, Kyoto 606-8501, Japan.
Abstract:
Number and size-controlled macromolecular associations are common in biology with viruses as a typical example. We report here a novel example of artificial viruses, in which the double-helical DNA is coated with 4-nm sized neutral glycocluster nanoparticles (GNPs). The resulting "glycoviruses" are compactly-packed, well charge-shielded (zeta approximately = 0 mV), and effectively transfect cell cultures without notable cytotoxicity.
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