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Updated: Aug 30, 2026
A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes
Published on: December 13, 2019
Spectrophotometry on measuring the size of liposomes
Guo-qin Niu1, Jun Pan, Wei-yue Lu
1Department of Pharmaceutics, School of Pharmacy, Fudan University, Shanghai 200032, China.
Aim:
To establish a spectrophotometric method for measurement of the sizes of liposomes for evaluating physical stability of liposomes.
Methods:
The sterically stabilized liposomes (SLs) were prepared by ethanol injection method and extrusion method. The mean cumulant diameters (D) of the vesicles were determined by electron microscopy and dynamic light scattering. On the basis of Rayleigh-Gans-Debye theory, the absorbance at 436 nm per unit lipid concentration (A436 nm/Cp) was measured as a function of vesicle diameter.
Results:
log(A436 nm/Cp) was closely related to logD (r2 > or = 0.93, n = 5).
Conclusion:
The absorbance of liposomes reflect their relative sizes and can be used to evaluate physical stability of liposomes.
