Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

GCF2: expression and molecular analysis of repression.

Toshiki Rikiyama1, Joseph Curtis, Masaya Oikawa

  • 1Laboratory of Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Room 5002, Building 37, 37 Convent Drive MSC-4264, Bethesda, MD 20892-4264, USA.

Biochimica Et Biophysica Acta
|October 3, 2003
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Invasive carcinoma derived from a branch-duct intraductal papillary mucinous neoplasm without mural nodules that developed after a long-term follow-up: a case report and review of literature].

Nihon Shokakibyo Gakkai zasshi = The Japanese journal of gastro-enterology·2026
Same author

The Vertebrate Genomes Project Phase I: A global reference genome resource.

bioRxiv : the preprint server for biology·2026
Same author

Immunoediting restricts clonal neoantigens in primary, treatment-naive human tumors.

Immunity·2026
Same author

The Utility of Intraoral Scanning and a Modifiable Milled Trial Denture in Treating Complete Edentulism in a Patient With Special Care Needs: A Case Report.

Special care in dentistry : official publication of the American Association of Hospital Dentists, the Academy of Dentistry for the Handicapped, and the American Society for Geriatric Dentistry·2026
Same author

Visualization of Scattered Tumor Regression Following Neoadjuvant Chemoimmunotherapy With a Novel Intraoperative PET/CT Scanner.

Head & neck·2026
Same author

Photobiomodulation in Treatment of Head and Neck Cancer-Related Chronic Lymphedema: A Pilot Randomized Controlled Trial.

Integrative cancer therapies·2026

GC-binding factor 2 (GCF2) acts as a transcriptional repressor, decreasing epidermal growth factor receptor (EGFR) activity. This gene is overexpressed in cancer cell lines, suggesting a role in cancer development.

Area of Science:

  • Molecular Biology
  • Genetics
  • Cancer Research

Background:

  • GC-binding factor 2 (GCF2) is identified as a transcriptional repressor impacting epidermal growth factor receptor (EGFR) and other gene activities.
  • Understanding the genetic and expression characteristics of GCF2 is crucial for its role in cellular regulation.

Purpose of the Study:

  • To map the GCF2 gene.
  • To analyze the structure and expression patterns of GCF2 mRNA and protein.
  • To investigate the functional role of GCF2 in transcriptional repression, particularly concerning the EGFR promoter.

Main Methods:

  • Fluorescence in situ hybridization (FISH) for gene mapping.
  • DNA sequencing for gene structure analysis.
  • Northern and Western blot analyses for gene and protein expression profiling.

Related Experiment Videos

  • Deletion mutant analysis to identify functional domains.

    • Main Results:

    • The GCF2 gene was mapped to chromosome 2q37.
    • GCF2 gene comprises eight exons spanning 73 kbp; a 4.2 kb mRNA transcript is ubiquitously expressed, with higher levels in leukocytes and significantly elevated in cancer cell lines, notably Burkitt's lymphoma.
    • GCF2 protein is highly expressed in cancer cell lines and localizes to both nuclear and cytoplasmic compartments. Amino acids 429-528 are essential for DNA binding and repression of the EGFR promoter, and GCF2 inhibits AP2-mediated EGFR promoter enhancement.

    Conclusions:

    • GCF2 is a transcriptional repressor localized to chromosome 2q37.
    • Differential expression of GCF2, particularly its overexpression in cancer cell lines, highlights its potential role in oncogenesis.
    • GCF2's ability to repress EGFR promoter activity and inhibit AP2 enhancement underscores its significance in regulating EGFR signaling pathways in cancer.