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Fetal tissue engineering: in vitro analysis of muscle constructs
Julie R Fuchs1, Irina Pomerantseva, Erin R Ochoa
1Harvard Center for Minimally Invasive Surgery, Harvard Medical School, Center for the Integration of Medicine and Innovative Technologies, Massachusetts General Hospital; and Children's Hospital, Boston, MA 02115, USA.
Journal of Pediatric Surgery
|October 3, 2003
Summary
Engineered fetal muscle constructs show rapid myoblast growth. Polyglycolic acid/poly-l-lactic acid and collagen hydrogel scaffolds support better cell attachment than polyglycolic acid/poly-4-hydroxybutyrate, impacting construct architecture.
Area of Science:
- Tissue Engineering
- Biomaterials Science
- Regenerative Medicine
Background:
- Fetal skeletal muscle myoblasts offer potential for tissue regeneration.
- Understanding scaffold interactions is crucial for successful muscle construct development.
Purpose of the Study:
- To evaluate the impact of various tissue engineering scaffolds on fetal muscle construct architecture.
- To compare cell attachment and proliferation on different biomaterials.
Main Methods:
- Ovine fetal myoblasts were cultured and seeded onto polyglycolic acid/poly-l-lactic acid, polyglycolic acid/poly-4-hydroxybutyrate, and collagen hydrogel scaffolds.
- Constructs were analyzed using histology, scanning electron microscopy, and DNA assays.
- Statistical analysis included likelihood ratio and paired Student's t tests.
Main Results:
- Fetal myoblasts exhibited faster proliferation rates than neonatal cells.
- Enhanced cell attachment was observed on polyglycolic acid/poly-l-lactic acid and collagen hydrogel scaffolds compared to polyglycolic acid/poly-4-hydroxybutyrate.
- Central necrosis within constructs correlated with increased cell seeding density and bioreactor duration.
Conclusions:
- Fetal myoblasts readily expand in culture and demonstrate good adherence to polyglycolic acid/poly-l-lactic acid and collagen hydrogel.
- Polyglycolic acid/poly-4-hydroxybutyrate showed suboptimal cell attachment.
- Optimizing cell density and bioreactor time is essential to prevent architectural degradation in engineered muscle constructs for future in vivo applications.