Fetal tissue engineering: in vitro analysis of muscle constructs
Related Experiment Video
Updated: Aug 30, 2026
Engineering Skeletal Muscle Tissues from Murine Myoblast Progenitor Cells and Application of Electrical Stimulation
Published on: March 19, 2013
Julie R Fuchs1, Irina Pomerantseva, Erin R Ochoa
1Harvard Center for Minimally Invasive Surgery, Harvard Medical School, Center for the Integration of Medicine and Innovative Technologies, Massachusetts General Hospital; and Children's Hospital, Boston, MA 02115, USA.
Background/Purpose:
This study was aimed at examining the impact of different tissue engineering techniques on fetal muscle construct architecture.
Methods:
Myoblasts from ovine specimens of fetal skeletal muscle were expanded in culture and their growth rates determined. Cells were seeded at different densities onto 3 scaffold types, namely polyglycolic acid (PGA) treated with poly-l-lactic acid (PLLA), a composite of PGA with poly-4-hydroxybutyrate (P4HB), and a collagen hydrogel. Constructs were maintained in a bioreactor and submitted to histologic, scanning electron microscopy, and DNA analyses at different time-points. Statistical analysis was by the likelihood ratio and paired Student's t tests (P <.05).
Results:
Fetal myoblasts proliferated at faster rates than expected from neonatal cells. Cell attachment was enhanced in the PGA/PLLA matrix and collagen hydrogel when compared with the PGA/P4HB composite. Necrosis was observed at the center of all constructs, directly proportional to cell seeding density and time in the bioreactor.
Conclusions:
Fetal myoblasts can be expanded rapidly in culture and attach well to PGA/PLLA, as well as collagen hydrogel but less optimally to PGA/P4HB. Excessive cell seeding density and bioreactor time may worsen final construct architecture. These findings should be considered during in vivo trials of muscle replacement by engineered fetal constructs.
