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Updated: Aug 30, 2026
A11-positive β-amyloid Oligomer Preparation and Assessment Using Dot Blotting Analysis
Published on: May 22, 2018
[Inhibiting effect of ethanol extract from Achyranthes bidentata on A beta 42 aggregation]
Huanmin Luo1, Feng Gu, Xiaoguang Li
1Pharmacy College, Jinan University, Guangzhou 510632.
Objective:
To observe inhibiting effect of ethanol extract from Achyranthes bidentata (Nx-E) on A beta 42 on (beta-amyloid protein 42) aggregation and fibril formation.
Methods:
A beta 42 and Nx-E were incubated in 37 degrees C, 5%, CO2 incubator first, fibril formation was monitored by thioflavine-T fluorescence and was observed with transmission electron microscopy.
Results:
At 7 days, 1 microgram.microL-1 A beta 42 fluorescence intensity was significantly increased, and much amyloid-like fibril was observed. A beta 42 co-incubated with 15 and 30 micrograms.microL-1 Nx-E for 7 days, their fluorescence intensities were remarkably reduced, few fibrils but an amorphous material were often observed.
Conclusions:
After 1 microgram.microL-1 A beta 42 was incubated alone up to 7 days, A beta 42 aggregated and amyloid-like fibril formed. 15 and 30 micrograms.microL-1 Nx-E can prevent A beta 42 from polymerizing to some extent.
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