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Updated: Aug 30, 2026

In Vivo Vascular Injury Readouts in Mouse Retina to Promote Reproducibility
Published on: April 21, 2022
Diattenuation and polarization preservation of retinal nerve fiber layer reflectance
Xiang-Run Huang1, Robert W Knighton
1Bascom Palmer Eye Institute, University of Miami School of Medicine, PO Box 016880, Miami, Florida 33101-6880, USA.
Abstract:
The diattenuation spectrum of the retinal nerve fiber layer (RNFL) reflectance has been predicted to depend strongly on the relative refractive index (m) of light-scattering cylinders. To constrain the values of m, diattenuation of the RNFL reflectance of isolated rat retina was measured with a multispectral imaging micropolarimeter. The RNFL reflection has very weak intrinsic diattenuation at all wavelengths (400-830 nm), which rejects all values of m > or = 1.03. Degree of polarization (DOP) for reflection from the RNFL was also measured. DOP was close to unity at all wavelengths, which indicates that the RNFL is a polarization-preserving reflector.
