Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Small interfering RNA: a new tool for gene therapy.

Feng Bi1, Na Liu, Daiming Fan

  • 1Institute of Digestive Diseases, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi Province, 710032, P.R. China. fengbi@fmmu.edu.cn

Current Gene Therapy
|October 8, 2003
PubMed
Summary

Small interfering RNAs (siRNAs) offer a novel approach to gene therapy by directly disrupting disease-related gene expression in mammalian cells. This RNA interference (RNAi) technology shows promise as a new class of anti-gene medicines.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Association between non-variceal spontaneous portosystemic shunt and outcomes after TIPS in cirrhosis.

Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·2018
Same author

Integrative Analysis of Dysregulated lncRNA-Associated ceRNA Network Reveals Functional lncRNAs in Gastric Cancer.

Genes·2018
Same author

Forkhead box C1 promotes colorectal cancer metastasis through transactivating ITGA7 and FGFR4 expression.

Oncogene·2018
Same author

Microbiota transplantation: concept, methodology and strategy for its modernization.

Protein & cell·2018
Same author

Nicotine induces aberrant hypermethylation of tumor suppressor genes in pancreatic epithelial ductal cells.

Biochemical and biophysical research communications·2018
Same author

Epithelial-to-Mesenchymal Transition: Liaison between Cancer Metastasis and Drug Resistance.

Critical reviews in oncogenesis·2018

Area of Science:

  • Molecular Biology
  • Gene Therapy
  • Biotechnology

Background:

  • Gene expression underlies numerous diseases, including neoplasms.
  • Antisense oligonucleotides and ribozymes are traditional anti-gene agents.
  • RNA interference (RNAi) is an emerging anti-gene technology.

Purpose of the Study:

  • To explore RNA interference (RNAi) as a therapeutic strategy.
  • To investigate the potential of small interfering RNAs (siRNAs) in gene therapy.

Main Methods:

  • Utilizing double-stranded RNA (dsRNA) to induce RNA interference.
  • Investigating the direct induction of RNAi by small interfering RNAs (siRNAs) in mammalian cells.

Main Results:

  • RNA interference (RNAi) involves processing of double-stranded RNA (dsRNA) to disrupt gene expression.

Related Experiment Videos

  • Small interfering RNAs (siRNAs), approximately 21-23 nucleotides, can directly induce RNAi in mammalian cells.
  • siRNAs demonstrate high specificity and efficiency in gene silencing.

    • Conclusions:

    • Small interfering RNAs (siRNAs) represent a promising anti-gene technology.
    • siRNAs have the potential to be developed as a new class of anti-gene medicines.
    • siRNAs may offer novel gene therapy applications for various diseases.