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Updated: Aug 30, 2026

Epon Post Embedding Correlative Light and Electron Microscopy
Published on: January 12, 2024
Formats of image data files that can be used for routine digital light micrography. Part one
1The Ludwig Institute for Cancer Research, 91, Riding House Street, London W1W 7BS, United Kingdom. alane@ludwig.ucl.ac.uk
Abstract:
Failing to open computer files that describe image data is not the most frustrating experience that the user of a computer can suffer, but it is high on list of possible aggravations. To ameliorate this, the structure of uncompressed image data files is described here. The various ways in which information that describes a picture can be recorded are related, and a primary distinction between raster or bitmap based and vector or object based image data files is drawn. Bitmap based image data files are the more useful of the two formats for recording complicated images such as digital light micrographs, whereas object based files are better for recording illustrations and cartoons. Computer software for opening a very large variety of different formats of digital image data is recommended, and if these fail, ways are described for opening bitmap based digital image data files whose format is unknown.
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