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Epon Post Embedding Correlative Light and Electron Microscopy
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Formats of image data files that can be used in routine digital light micrography. Part two
1The Ludwig Institute for Cancer Research, 91 Riding House Street, London W1W 7BS, United Kingdom. alane@ludwig.ucl.ac.uk
Abstract:
This is part two of an article that describes the properties of the image data files that are encountered routinely in digital light micrography. In the current part of the article, the differences between saving image data as large intact files and smaller files that have had some information removed, i.e., using lossy compression, are related first. Subsequently, appropriate ways of configuring computers to deal with the large intact image data files are suggested. The structures of the image data files used for recording dynamic sequences and kinematic animations of series of digital light micrographs, i.e., movie formats, are then described. Finally, some information is supplied about choosing file formats for compressing both static and dynamic image data sets.
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