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Measurement of T Cell Alloreactivity Using Imaging Flow Cytometry
Published on: April 19, 2017
Immunophenotyping of angioimmunoblastic T-cell lymphomas by multiparameter flow cytometry
Po-Shing Lee1, Ching-Nan Lin, Shih-Sung Chuang
1Department of Pathology, Chi-Mei Medical Center, Yungkang City, Tainan, Taiwan.
Pathology, Research and Practice
|October 10, 2003
Summary
Flow cytometry can now aid in diagnosing Angioimmunoblastic T-cell lymphoma (AITL). Researchers found CD4+/CD10+ T-cells in AITL cases, suggesting a new diagnostic marker for this lymphoma.
Area of Science:
- Hematology
- Immunology
- Oncology
Background:
- Angioimmunoblastic T-cell lymphoma (AITL) is a peripheral T-cell lymphoma (PTCL) with systemic symptoms.
- Histologic diagnosis of AITL can be challenging due to subtle early-phase features.
- Flow cytometry (FCM) is not widely established for PTCL diagnosis, unlike its success in B-cell neoplasms.
Observation:
- Recent studies indicate neoplastic T-cells in AITL can express CD10.
- This study prospectively applied multiparameter FCM to three AITL cases.
- A small population of CD4+/CD10+ T-cells was identified in two AITL cases.
Findings:
- The CD4+/CD10+ T-cell population exhibited variable expression of CD3, CD7, CD2, and CD45.
- This specific CD4+/CD10+ T-cell population was absent in reactive lymph nodes and other lymphomas.
- The presence of CD4+/CD10+ T-cells suggests a potential diagnostic marker for AITL.
Implications:
- Adding CD4/CD10 and CD3/CD10 markers to FCM panels may improve AITL diagnosis.
- This research is the first to report CD10-expressing T-cells in AITL using FCM.
- Enhanced diagnostic accuracy for AITL can lead to timely and appropriate patient management.
