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Selective fluorescence quenching of 2,3-diazabicyclo[2.2.2]oct-2-ene by nucleotides

Cesar Marquez1, Uwe Pischel, Werner M Nau

  • 1School of Engineering and Science, International University Bremen, Campus Ring 1, D-28759 Bremen, Germany.

Organic Letters
|October 11, 2003
PubMed

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Restriction Enzymes01:11

Restriction Enzymes

Restriction enzymes are bacterial enzymes used to cut DNA in a sequence-specific manner. To cleave DNA, they bind to specific palindromic sequences called restriction sites. Such palindromic DNA sequences or inverted repeats are commonly found in regions of functional significance, such as the origin of replication, gene operator sites, and regions containing transcription termination signals.
The host bacteria protect their own genomic DNA from these enzymes by methylating these sites. Some...