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Updated: Aug 30, 2026

Nuclear Transfer into Mouse Oocytes
Published on: November 30, 2006
Reprogramming in nuclear transfer
Alice Jouneau1, Jean-Paul Renard
1Unité Mixte de Recherche, Biologie du Développement et Reproduction, Bât 440, Institut National de la Recherche Agronomique, 78350, Jouy-en-Josas, France. jouneau@jouy.inra.fr
Abstract:
Reprogramming a nucleus by transferring it to oocyte cytoplasm triggers epigenetic changes that eventually lead to the restoration of a totipotent state and the birth of a viable animal. A simplified way of studying this complex process is to study cell hybrids. These studies suggest that the pluripotent character of one nucleus is dominant over that of the other nucleus. The development of nuclear transfer embryos shows that there are several restriction points and that the extra-embryonic lineages may be the primary source of death. Successful reprogramming depends on the balance between epigenetic modifications and the regulative properties of development.
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